ISSN; 2411-1376

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Volume 4 Issue 2

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Original research article from Osmania University, Hyderabad, India

Stability–indicating RP-HPLC method for the determination of Metformin and Dapagliflozin in  bulk and pharmaceutical dosage form
      N.Padmaja and G.Veerabhadram

    A simple and more economic isocratic  stability-indicating RP-HPLC method was developed and subsequently validated  for the simultaneous determination of Metformin and Dapagliflozin in bulk and pharmaceutical dosage form. The chromatographic conditions were standardized using a Themosil C18HPLC column with 150mm in length and internal diameter of 4.6mm with size 5µm.The analyte detection was carried out by using a PDA detector set at a wavelength of 234 nm. The mobile phase consisted of 0.1M orthophosphoric acid: acetonitrile:methanol (35:40:25v/v/v).The retention time was found to be 2.102 minutes and 4.105 minutes for Metformin and Dapagliflozin respectively. The calibration curves of two drugs were linear with correlation coefficients of 0.999 and 0.999 over a concentration range of 0.5-2.5µg/ml   for Metformin and 5-25µg/ml for Dapagliflozin. This method has been validated and shown to be accurate, precise, specific, sensitive, linear, robust and fast. Metformin and Dapagliflozin were subjected to different degradation stress conditions. The degradation products were well resolved from that of pure standard drugs (Metformin and Dapagliflozin) with significant different retention time values. The current method has been statistically validated according to the ICH guidelines and this method has been subsequently developed and applied successfully to determine the levels of Metformin and Dapagliflozin in a combined formulation and in the routine quality control analysis with good accuracy and sensitivity.

 Key words: Metformin, Dapagliflozin, RP-HPLC, Method development, Validation.


Original research article from University of Santo Tomas Graduate School, Philippines

Determining the Significance of the Philippine Sin Tax Law on University Students’ Smoking Behavior
         Marie Antoinette L. Rosete

    The Philippine Sin Tax Law (STL) was enforced in 2013 and restructured the excise taxes imposed on tobacco products. It was anticipated that higher cigarette prices would lead to reduced consumption by the long-addicted while potential smokers become discouraged by the high prices to even start. The Department of Health reported reduced cigarette consumption within subpopulations but national smoking prevalence has yet to notably decrease. This research looked into whether there was a significant change in the cigarette-purchasing and smoking behavior among Filipino university students. Primary data from a survey questionnaire with a causal-comparative design was fielded among the University of Santo Tomas College of Commerce and Business Administration Business Economics majors during the AY2015-2016 was utilized and analyzed via a paired difference test to establish if the change in cigarette prices before and after STL as well as the change in the respondents’ daily consumption were significant or otherwise. Results showed that there was a significant difference in cigarette prices after STL but there was no significant difference in cigarette consumption.  This research was able to show how the individual eventually copes with the price change given that he/she is already addicted to nicotine. Policy improvements along the lines of encouraging both youth smoking cessation and non-take-up are recalculating retail prices to check for affordability as well as restrictions on single stick retail practices.

Key words: Philippine Sin Tax Law, Smoking Behavior, tobacco products, Filipino university students

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